Plot Tips
# %pip install pycirclize
Subplots¶
pyCirclize supports plotting to user-defined Polar Axes. Users can target subplot Polar Axes with the circos.plotfig()
method to combine them with other plots.
from pycirclize import Circos
import matplotlib.pyplot as plt
import numpy as np
import pandas as pd
np.random.seed(0)
# Create 4 subplots
fig = plt.figure(figsize=(8, 8), dpi=150)
ax1 = fig.add_subplot(221)
polar_ax2 = fig.add_subplot(222, polar=True)
polar_ax3 = fig.add_subplot(223, polar=True)
polar_ax4 = fig.add_subplot(224, polar=True)
# Plot to first subplot (ax1)
x = np.linspace(0, 2 * np.pi, 100)
ax1.plot(x, np.sin(x))
# Plot circos to second subplot (polar_ax2)
sectors = {"A": 10, "B": 20, "C": 15}
circos = Circos(sectors, space=10)
for sector in circos.sectors:
track = sector.add_track((80, 100), r_pad_ratio=0.1)
track.axis()
track.xticks_by_interval(5)
vmin, vmax = 0, 10
# Line between start-end two points
track.line([track.start, track.end], [vmin, vmax], lw=1, ls="dotted")
# Line of random value points
x = np.linspace(track.start, track.end, int(track.size) * 5 + 1)
y = np.random.randint(vmin, vmax, len(x))
track.line(x, y)
fig = circos.plotfig(ax=polar_ax2)
# Plot chord diagram to fourth subplot (polar_ax4)
row_names = ["F1", "F2", "F3"]
col_names = ["T1", "T2", "T3", "T4", "T5", "T6"]
matrix_data = [
[4, 14, 13, 17, 5, 2],
[7, 1, 6, 8, 12, 15],
[9, 10, 3, 16, 11, 18],
]
matrix_df = pd.DataFrame(matrix_data, index=row_names, columns=col_names)
circos = Circos.initialize_from_matrix(
matrix_df,
r_lim=(95, 100),
space=5,
cmap="tab10",
link_kws=dict(ec="black", lw=0.5, direction=1),
)
fig = circos.plotfig(ax=polar_ax4)
Grouping Sectors¶
In pyCirclize, the space size between sectors can be set arbitrarily.
By using the calc_group_spaces
function, users can easily get the sector space size setting
within/between groups based on the number of groups.
Groups can be explicitly visualized as shown in the code example below.
from pycirclize import Circos
from pycirclize.utils import calc_group_spaces, ColorCycler
sectors = {name: 10 for name in "ABCDEFGHIJ"}
group1, group2, group3 = list("ABCD"), list("EFG"), list("HIJ")
# Calculate appropriate group between/within spaces
# In this example, 10 sectors are displayed as groups dividied into [4, 3, 3]
spaces = calc_group_spaces([4, 3, 3], space_bw_group=15, space_in_group=2)
circos = Circos(sectors, space=spaces)
# Plot sector track
ColorCycler.set_cmap("Set3")
for sector in circos.sectors:
track = sector.add_track(r_lim=(85, 90))
track.axis(fc=ColorCycler())
track.text(sector.name)
# Plot group sectors range
ColorCycler.set_cmap("tab10")
for idx, group in enumerate((group1, group2, group3), 1):
group_deg_lim = circos.get_group_sectors_deg_lim(group)
circos.rect(r_lim=(97, 100), deg_lim=group_deg_lim, fc=ColorCycler(), ec="black", lw=0.5)
group_center_deg = sum(group_deg_lim) / 2
circos.text(f"Group{idx}", r=105, deg=group_center_deg, adjust_rotation=True)
fig = circos.plotfig()
Anti-clockwise¶
pyCirclize sets the coordinate direction of each sector as clockwise by default.
Users can set the coordinate direction of any sector to anti-clockwise with
sector2clockwise
option when initializing Circos instance.
from pycirclize import Circos
sectors = dict(A1=18000, A2=13000, A3=6000, A4=5000, B3=12000, B2=13000, B1=15000)
circos = Circos(
sectors,
space=3,
# Set (B1, B2, B3) sector coordinate direction as anti-clockwise
sector2clockwise=dict(B1=False, B2=False, B3=False),
)
for sector in circos.sectors:
# Plot track
color = "orange" if sector.name.startswith("A") else "skyblue"
track = sector.add_track((94, 100), r_pad_ratio=0.1)
track.axis(fc=color)
track.text(sector.name)
# Plot xticks
major_interval, minor_interval = 5000, 1000
track.xticks_by_interval(major_interval, label_orientation="vertical", label_formatter=lambda v: f"{v / 1000:.0f} Kb")
track.xticks_by_interval(minor_interval, tick_length=1, show_label=False)
# Plot links
circos.link(("A1", 0, 10000), ("B1", 0, 10000))
circos.link(("A1", 18000, 13000), ("B1", 10000, 15000), color="tomato")
circos.link(("A2", 0, 13000), ("B2", 0, 13000))
circos.link(("A3", 0, 6000), ("B3", 6000, 12000))
circos.link(("A4", 0, 5000), ("B3", 0, 5000))
fig = circos.plotfig()
Colorbar¶
Users can easily plot colorbars with circos.colorbar()
method.
from pycirclize import Circos
import numpy as np
np.random.seed(0)
# Initialize Circos instance
circos = Circos(sectors=dict(data=100), start=90)
sector = circos.sectors[0]
# Plot heatmap track1 (cmap="bwr")
track1 = sector.add_track((85, 100))
track1.axis()
vmin1, vmax1, cmap1 = 0, 100, "bwr"
matrix1 = np.random.randint(vmin1, vmax1, (5, 100))
track1.heatmap(matrix1, cmap=cmap1, vmin=vmin1, vmax=vmax1)
# Plot heatmap track2 (cmap="viridis")
track2 = sector.add_track((65, 80))
track2.axis()
vmin2, vmax2, cmap2 = -200, 200, "viridis"
matrix2 = np.random.randint(vmin2, vmax2, (3, 100))
track2.heatmap(matrix2, cmap=cmap2, vmin=vmin2, vmax=vmax2)
# Plot colorbar in various style
# bounds = (x, y, width, height)
circos.colorbar(vmin=vmin1, vmax=vmax1)
circos.colorbar(bounds=(0.7, 0.6, 0.02, 0.3), vmin=vmin1, vmax=vmax1, cmap="bwr")
circos.colorbar(bounds=(0.8, 0.6, 0.02, 0.3), vmin=vmin2, vmax=vmax2, cmap="viridis")
circos.colorbar(
bounds=(0.3, 0.485, 0.4, 0.03),
vmin=vmin2,
vmax=vmax2,
cmap="viridis",
orientation="horizontal",
label="Colorbar in center",
label_kws=dict(size=12, color="blue"),
tick_kws=dict(labelsize=12, colors="red"),
)
fig = circos.plotfig()
Legend¶
Example of manual legend plotting code using Axes.legend()
method.
See Legend guide for more details.
from pycirclize import Circos
from pycirclize.utils import ColorCycler
from matplotlib.patches import Patch
from matplotlib.lines import Line2D
import numpy as np
np.random.seed(0)
line_colors = ("red", "blue", "green")
scatter_colors = ("orange", "purple", "black")
scatter_markers = ("o", "^", "+")
cmap_name = "tab10"
sectors = dict(A=10, B=15, C=12)
circos = Circos(sectors, space=5)
for sector in circos.sectors:
sector.text(sector.name, r=110, size=12)
# Plot line track
track1 = sector.add_track((80, 100), r_pad_ratio=0.1)
track1.axis()
track1.xticks_by_interval(interval=1)
for line_color in line_colors:
x = np.arange(sector.start, sector.end) + 0.5
y = np.random.randint(0, 100, len(x))
track1.line(x, y, color=line_color)
# Plot scatter track
track2 = sector.add_track((55, 75), r_pad_ratio=0.1)
track2.axis()
for color, marker in zip(scatter_colors, scatter_markers):
point_num = 10
x = np.random.rand(point_num) * (sector.end - 1) + 0.5
y = np.random.rand(point_num) * 10
track2.scatter(x, y, c=color, marker=marker, s=15)
# Plot rect track
track3 = sector.add_track((47, 50))
ColorCycler.set_cmap(cmap_name)
for idx in range(int(sector.start), int(sector.end)):
track3.rect(idx, idx + 1, color=ColorCycler(), ec="black", lw=0.5)
fig = circos.plotfig()
# Plot legend
line_handles = [
Line2D([], [], color="red", label="Line 01"),
Line2D([], [], color="blue", label="Line 02"),
Line2D([], [], color="green", label="Line 03"),
]
line_legend = circos.ax.legend(
handles=line_handles,
bbox_to_anchor=(0.93, 1.02),
fontsize=8,
title="Lines",
handlelength=2,
)
circos.ax.add_artist(line_legend)
scatter_handles = [
# Marker List: https://matplotlib.org/stable/api/markers_api.html
Line2D([], [], color="orange", marker="o", label="Point 01", ms=6, ls="None"),
Line2D([], [], color="purple", marker="^", label="Point 02", ms=6, ls="None"),
Line2D([], [], color="black", marker="+", label="Point 03", ms=6, ls="None"),
]
scatter_legend = circos.ax.legend(
handles=scatter_handles,
bbox_to_anchor=(0.93, 0.90),
fontsize=8,
title="Points",
handlelength=2,
)
circos.ax.add_artist(scatter_legend)
rect_handles = []
rect_colors = ColorCycler.get_color_list()
for idx, color in enumerate(rect_colors, 1):
rect_handles.append(Patch(color=color, label=f"Type {idx:02d}"))
_ = circos.ax.legend(
handles=rect_handles,
bbox_to_anchor=(0.5, 0.5),
loc="center",
fontsize=8,
title="Types",
ncol=2,
)
# *** To save a figure with legend as a file, use `fig.savefig()` method ***
# *** `circos.savefig()` method does not save the figure with legend ***
# fig.savefig("result.png", dpi=100)
Stacked Bar¶
Users can easily plot stacked bar chart from matrix dataframe with row and column names.
Vertical and horizontal stacked bar chart can be plotted using track.stacked_bar()
and track.stacked_barh()
methods, respectively.
from pycirclize import Circos
from matplotlib.patches import Patch
import pandas as pd
import numpy as np
np.random.seed(0)
# Generate matrix data for stacked bar plot
row_num, col_num = 12, 6
matrix = np.random.randint(5, 20, (row_num, col_num))
row_names = [f"R{i}" for i in range(row_num)]
col_names = [f"group{i}" for i in range(col_num)]
table_df = pd.DataFrame(matrix, index=row_names, columns=col_names)
print(table_df)
# Initialize Circos sector & track
circos = Circos(sectors=dict(bar=len(table_df.index)))
sector = circos.sectors[0]
track = sector.add_track((50, 100))
track.grid()
# Plot stacked bar
colormap = "Set3"
sb_table = track.stacked_bar(
table_df,
width=0.6,
cmap=colormap,
bar_kws=dict(ec="black", lw=0.2),
label_pos="bottom",
label_kws=dict(size=10, orientation="horizontal"),
)
fig = circos.plotfig()
# Plot legends
col_name2color = sb_table.get_col_name2color(colormap)
_ = circos.ax.legend(
handles=[Patch(label=n, fc=c, ec="black", lw=0.5) for n, c in col_name2color.items()],
bbox_to_anchor=(0.5, 0.5),
loc="center",
ncols=2,
)
# fig.savefig("stacked_bar.png")
group0 group1 group2 group3 group4 group5 R0 17 10 5 8 16 8 R1 12 14 8 10 7 9 R2 12 11 13 13 17 15 R3 6 11 12 12 19 13 R4 6 10 14 18 13 14 R5 9 8 5 8 10 19 R6 5 7 8 13 6 8 R7 18 8 8 19 12 5 R8 6 14 14 5 15 9 R9 12 8 19 16 7 12 R10 17 7 5 5 9 10 R11 10 11 13 9 6 9
from pycirclize import Circos
from pycirclize.parser import StackedBarTable
from matplotlib.patches import Patch
import pandas as pd
import numpy as np
np.random.seed(0)
# Generate & load matrix data for horizontal stacked bar plot
row_names = list("ABCDEF")
col_names = ["group1", "group2", "group3", "group4", "group5", "group6"]
matrix = np.random.randint(5, 20, (len(row_names), len(col_names)))
table_df = pd.DataFrame(matrix, index=row_names, columns=col_names)
sb_table = StackedBarTable(table_df)
print(table_df)
# Initialize Circos sector & track (0 <= range <= 270)
circos = Circos(sectors=dict(bar=sb_table.row_sum_vmax), start=0, end=270)
sector = circos.sectors[0]
track = sector.add_track((30, 100))
track.axis(fc="lightgrey", ec="black", alpha=0.5)
# Plot horizontal stacked bar & label & xticks
colormap = "tab10"
track.stacked_barh(sb_table.dataframe, cmap=colormap, width=0.6)
label_r_list = sb_table.calc_barh_label_r_list(track.r_plot_lim)
for label_r, row_name in zip(label_r_list, sb_table.row_names):
track.text(f"{row_name} ", x=0, r=label_r, ha="right")
track.xticks_by_interval(interval=5)
track.xticks_by_interval(interval=1, tick_length=1, show_label=False)
fig = circos.plotfig()
# Plot legends
col_name2color = sb_table.get_col_name2color(colormap)
_ = circos.ax.legend(
handles=[Patch(label=n, fc=c, ec="black", lw=0.5) for n, c in col_name2color.items()],
bbox_to_anchor=(0.2, 0.8),
fontsize=12,
loc="center",
ncols=2,
)
# fig.savefig("stacked_barh.png")
group1 group2 group3 group4 group5 group6 A 17 10 5 8 16 8 B 12 14 8 10 7 9 C 12 11 13 13 17 15 D 6 11 12 12 19 13 E 6 10 14 18 13 14 F 9 8 5 8 10 19
Appendix¶
1. Clock¶
from pycirclize import Circos
import numpy as np
circos = Circos(sectors={"clock": 12})
sector = circos.sectors[0]
# Plot outer line & xticks
track = sector.add_track(r_lim=(100, 100))
major_xticks = np.arange(0, 12, 1) + 1
track.xticks(major_xticks, outer=False, show_bottom_line=True)
minor_xticks = np.arange(0, 12, 0.2)
track.xticks(minor_xticks, outer=False, tick_length=1)
# Plot 1-12 hour characters
for x in major_xticks:
track.text(str(x), x=x, r=93, size=15, adjust_rotation=False)
# Plot clock hands (hour, minute, second)
sector.line(r=(0, 40), start=8, end=8, lw=3)
sector.line(r=(0, 70), start=0, end=0, lw=1.5)
sector.line(r=(0, 80), start=5, end=5, lw=0.5, color="red")
fig = circos.plotfig()
2. Dartboard¶
from pycirclize import Circos
points = [20, 1, 18, 4, 13, 6, 10, 15, 2, 17, 3, 19, 7, 16, 8, 11, 14, 9, 12, 5]
sectors = {str(p): 1 for p in points}
circos = Circos(sectors, space=0, start=-9, end=351)
red_or_green = ("red", "green")
black_or_white = ("black", "white")
circos.rect(r_lim=(85, 100), fc="black")
for idx, sector in enumerate(circos.sectors):
sector.text(sector.name, r=92.5, color="white", size=12, adjust_rotation=False)
color_idx = idx % 2
sector.rect(r_lim=(80, 85), fc=red_or_green[color_idx], ec="lightgrey", lw=1)
sector.rect(r_lim=(52.5, 80), fc=black_or_white[color_idx], ec="lightgrey", lw=1)
sector.rect(r_lim=(47.5, 52.5), fc=red_or_green[color_idx], ec="lightgrey", lw=1)
sector.rect(r_lim=(10, 47.5), fc=black_or_white[color_idx], ec="lightgrey", lw=1)
circos.rect(r_lim=(5, 10), fc="green")
circos.rect(r_lim=(0, 5), fc="red")
circos.line(r=5, color="lightgrey", lw=1)
fig = circos.plotfig()